Analytical Ultracentrifugation of Polymers and Nanoparticles by Walter Maechtle

By Walter Maechtle

This ebook is split into chapters overlaying instrumentation, sedimentation pace runs, density gradient runs, program examples and destiny advancements. specifically, the distinctive software bankruptcy demonstrates the flexibility and gear of AUC by way of many attention-grabbing and demanding business examples. therefore the e-book concentrates on sensible facets instead of information of centrifugation theory.

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11] inside a Model E (reprinted with permission from [11]) 40 2 Analytical Ultracentrifugation, Instrumentation reflectance [12] or in transmission mode [11] by a PM tube. A filter is used in front of the photomultiplier to ensure that only fluorescence light passes and excitation light is omitted. Riesner et al. described the first fluorescence detector adapted to an AUC [11]. They used the geometry of the Schlieren optics channel of a Model E (see Fig. 11), and replaced the Model E components of the Schlieren optics by the components of the fluorescence detector.

8)), it will be shown for the case of equilibrium runs how the mass conservation law can be utilized to calculate J(rm ) and c(rm ). Again, the detecting system of the older Model E by Beckman served as basis for the development of the new XL-A/I interference optics [18, 19]. In comparison, major changes have been made to the light source and the detecting unit. The constantly burning mercury lamp as light source was replaced by a pulsed laser diode. A CCD camera replaced the photographic plate as detecting unit.

3 Schlieren Optics The Schlieren optics setup (as described above in Sect. 2 and Fig. 3) is similar to the setup of the Rayleigh interferometer but has a phase plate (or knife-edge) in the focus of the condenser lens as an additional element. As there is no commercially available system anymore, users are obliged to construct their own Schlieren optics, or transfer the Schlieren optics of an older Model E onto the Optima XL platform [7]. As mentioned above, the driving force to do this comes from some unique advantages of Schlieren optics, of which two are given here: – It allows the use of simple mono-sector cells that are easy to handle, to tighten, and that assure highest reproducibility.

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